Edificio 25, Campus Universitario de Espinardo
30100 Murcia, Spain
Tuesday 17 May 2016
8:30 am – 4.30 pm
Project coordinator: Miguel A. Aranda, CEBAS-CSIC
Tuesday 17, May 2016 - Agenda
Attendees: Miguel A. Aranda, Miguel Juarez, Yolanda Hernando, Pedro Gómez López (CEBASCSIC), Anastasia Tsagkarakou (DEMETER), Maja Ravnikar (NIB), Bayram Çevik, Fevziye Celebi Toprak (ACPPRI-PAU-SDU), Fernando García-Arenal (CBGP-UPM), Mohamed Faize (UCDEJ), Moshe Lapidot (VC), Cécile Desbiez (INRA), Michel Peterschmitt, Cica Urbino (CIRAD), Laura Tomassoli, Sabrina Bertin (CREA).
Apologies: Sherin A. Mahfouze (NRC), Khalid A. El-Dougdoug (UAS).
Welcome to CEBAS-CSIC. Miguel A. Aranda.
Short presentations from partners
- Partner 1. CEBAS-CSIC. Miguel A. Aranda.
- Partner 2. DEMETER. Anastasia Tsagkarakou.
- Partner 3. NIB. Maja Ravnikar.
- Partner 4. ACPPRI-PAU-SDU. Bayram Çevik.
- Partner 5. CBGP-UPM. Fernando García-Arenal.
- Partner 8. UCDEJ. Mohamed Faize.
- Partner 9. VC. Moshe Lapidot.
- Partner 10. INRA. Cécile Desbiez.
- Partner 11. CIRAD. Cica Urbino.
- Partner 12 CRA. Laura Tomassoli.
Work packages descriptions and tasks assigned to each partner
- WP1. Project management and networking. Miguel A. Aranda.
- WP2. Mapping viruses and vectors in crops and reservoirs. Cécile Desbiez.
- WP3. Virus discovery and detection. Maja Ravnikar.
- WP4. Virus ecology and evolution. Fernando García-Arenal.
- WP5. Virus-host interactions. Miguel A. Aranda on behalf of Suat Kaymak/Fevziye Çelebi-
- WP6. Virus control. Moshe Lapidot.
- WP7. Technology transfer and dissemination. Yolanda Hernando.
Discussion of any issue raised and pending aspects
Short presentations from partners
Partners presented main research lines of their groups and their interests and commitments within the EMERAMB project. PowerPoint presentations are available to all partners, and will be posted online in the members area of the EMERAMB website.
WPs: Objectives and partners commitments within each WP
WP coordinators reviewed objectives and partners commitments within each WP. No major deviations from what was proposed could be foreseen. Surveys to compose the EMERAMB core collections received particular attention; conclusions are described in the following section.
WP2: Surveys and core collections
Cécile Desbiez (INRA) coordinates WP2. She pointed out the necessity to define more accurately how surveys to compose the EMERAMB isolates core collection are going to be performed. Upon discussion among partners, it was agreed that surveys should concentrate in melon and tomato crops. For each crop, each participant partner will collect, from symptomatic plants, 30 samples per year, with 3-6 samples per plot from a minimum of 5 plots at least 3-4 km apart. For each plot, GPS coordinates, and if possible some information on the crop (date of planting, cultivar, cover, plastic mulch or not, conventional/organic farming, irrigation or not, abundance of weeds and insects...) and
its surroundings (neighboring crops, abundance of weeds, landscape continuity or not) will be taken. Participant partners will use samples in their labs to detect the viruses of interest (see below). Samples positive for the viruses of interest will be shipped to participant partners (see below) who agreed to analyze specific aspects of the corresponding virus and whitefly populations.
Partners involved are: VC, CEBAS-CSIC, CREA, UCDEJ, ACPPRI-PAU-SDU, NIB, INRA.
Viruses diagnosed will be: WMV, CMV, ZYMV and CABYV.
Shipping material will be: RNA extracts.
INRA will analyze WMV, CMV, ZYMV populations.
CEBAS-CSIC will analyze CABYV populations.
Partners involved are: VC, CEBAS-CSIC, UCDEJ, ACPPRI-PAU-SDU, NIB, DEMETER, CIRAD.
Viruses diagnosed will be: TYLCV.
Shipping material will be: DNA extracts.
Whiteflies will also be sampled. From the same tomato plots surveyed for virus detection. 50
CIRAD will analyze TYLCV populations.
DEMETER will analyze whitefly populations.
Recommendations about sampling/shipping materials:
“heavy duty hand-held vac/aspirator”; http://www.bioquip.com/Search/DispProduct.asp?pid=2820GA.
“flashlight aspirator”; http://www.bioquip.com/Search/DispProduct.asp?pid=2809C.
Venue and date for the next meeting
It was agreed that Ljubljana would be a perfect location for the next meting, as it is central to all partners and well communicated. Maja Ravnikar was happy to host the meeting, and manifested the interest of coordinating this with other potential meetings, particularly those of a COST action that she is coordinating. Miguel A. Aranda proposed to held the meeting around June 2017, well before schedule, to be sure that, if any aspect on the development of the project needs to be corrected, the EMERAMB consortium would have enough time to do it.